The health calamity caused by obesity throughout the world is a burning issue in the sedentary lifestyle. Obesity heightens the risk of associated morbidities, including diabetes, hypertension, cancer, heart disease and so on. Overexpression of Protein tyrosine phosphatase 1B (PTP1B) enzyme is responsible for type 2 diabetes and obesity. Numerous anti-diabetic synthetic drugs are available in the market but not away from severe side effects. So, the research is focused on identification of the potent plants extracts that exhibit inhibitory activity against PTP1B enzyme. Regarding the goal, ten plants extracts were tested to inhibit the activity of PTP1B enzyme. Some of the extracts were showed remarkable inhibition percentage (60-80%) against PTP1B by pNPP assay.